Al Ain FC (UAE)
- 2023 AFC Champions League™ winners, their second title after winning the first edition of the ACL in 2003
- Only UAE side to win the ACL
- 17th ACL appearance
- 3rd in UAE Pro League 2023/24

Al Hilal SFC (KSA)
- Four-time Asian champions
- Reached a record five ACL Finals
- 2023/24 Saudi Pro League champions – record 19th title
- also won 2023/24 King’s Cup
- 19th ACL appearance, joint-record

Al Sadd SC (QAT)
- 2011 ACL champions – only Qatari club to win ACL
- 17th ACL appearance, 7th in a row
- 2023/24 Qatari Stars League champions – record 17th title
- also won 2023/24 Amir of Qatar Cup – record 19th title

Persepolis FC (IRN)
- 2023/24 IR Iran league champions – record 16th title
- 2-time ACL finalists
- 12th ACL appearance

Pakhtakor (UZB)
- 2023 Uzbekistan Super League champions – 5th in a row and record 16th title
- Two-time semi-finalists
- 19th ACL appearance, joint-record
- 6th consecutive ACL appearance

Al Shorta (IRQ)
- 2023/24 Iraq Stars League champions – 3rd title in a row
- Won Iraq FA Cup for first time in 2023/24
- 5th ACL appearance, first since 2021
- Never made it to KO stage of ACL

Al Nassr Club (KSA)
- 2023/24 Saudi Pro League runners-up
- 9-time league winners
- 8th ACL appearance
- 2-time semi-finalists

Al Rayyan SC (QAT)
- 2023/24 Qatar Stars League runners-up
- 8-time league winners
- 12th ACL appearance, first since 2022
- Reached KO Stage once

Esteghlal FC (IRN)
- 2023/24 IR Iran league runners-up
- 9-time league winners
- 13th ACL appearance, first since 2021
- 1-time semi-finalist (2013)

Al Wasl FC (UAE)
- 2023/24 UAE Pro League champions – 8th title and first since 2007
- Also won 2023/24 UAE President’s Cup – 3rd title and first since 2007
- 4th ACL appearance, first since 2019
- Have never reached KO Stage

Al Ahli Saudi FC (KSA)
- 3rd in 2023/24 Saudi Pro League
- 3-time league winners
- 2012 ACL finalists
- 13th ACL appearance, first since 2021

Al Gharafa SC (QAT)
- 3rd in 2023/23 Qatar Stars League
- 7-time Qatari league winners
- 1-time quarter-finalists (2010)
- 10th ACL appearance, first since 2022