Sanfrecce Hiroshima (JPN)
- 3rd in 2023 J1 League
- 3-time league winners
- 5 ACL appearances
- 1st ACL Two appearance

Sydney FC (AUS)
- 2023 Australia Cup champions, 2nd title
- Record 5-time A-League Men champions
- 7 ACL appearances
- 1st ACL Two appearance

Kaya FC-Iloilo (PHI)
- 2024 Philippines Football League champions – 2nd title and 2nd in a row
- 2023 PFL Cup winners – record 3rd title
- 1-time AFC Cup Round of 16 (2016)
- 4th ACL Two appearance

Eastern (HKG)
- 2023/24 Hong Kong FA Cup winners – 6th title
- 1-time league champions
- Reached inter-zone play-off semi-finals in 2022 AFC Cup
- 4th ACL Two appearance

Zhejiang FC (CHN)
- 3rd in 2023 Chinese Super League
- 2 ACL appearances
- 1st ACL Two appearance

Port FC (THA)
- 3rd in 2023/24 Thai League 1
- 1 ACL appearance
- 2nd ACL Two appearance, first came in 2010

Lion City Sailors FC (SGP)
- 2023 Singapore Premier League runners-up
- 1-time league champions
- 2023 Singapore Cup winners – 1st title
- 2 ACL appearances
- 1st ACL Two appearance

Persib Bandung (IDN)
-2023/24 Indonesia Liga 1 champions – 2nd title and 1st since 2014
- Reached Round of 16 in AFC Cup debut in 2015, their last appearance in AFC club competition
- 2nd ACL Two appearance

Bangkok United (THA)
- 2023/24 Thai FA Cup winners – first title
- 2023/24 Thai League 1 runners-up
- 1-time league winners (2006)
- 3 ACL appearances
- 1st ACL Two appearance

Nam Dinh FC (VIE)
- 2023/24 V League 1 winners – 2nd title and first since 1985
- 1st ACL Two appearance
- Only team in the draw set to make their AFC Club Competition debut

-2023/24 Hong Kong Premier League champions- 1st title
-2022/23 Hong Kong Premier League runners-up
-Two ACL Two appearances

Tampines Rovers FC (SGP)
- 3rd in 2023 Singapore Premier League
- 5-time league champions
- 4-time quarter-finalists in AFC Cup (2005, 2006, 2007, 2016)
- 13th ACL Two appearance

Jeonbuk Hyundai Motors FC (KOR)
- 4th in 2023 K League 1
- Record 9-time league champions
- 2-time ACL champions (2006, 2016)
- 16 ACL appearances, most by any Korean club
- 1st ACL Two appearance

 Selangor FC (MAS)
- 2023 Malaysia Super League runners-up
- 2-time league champions
- 1-time quarter-finalists (2006)
- Returning to AFC club competition for first time since 2016 AFC Cup
- 6th ACL Two appearance

Muangthong United (THA)
- 5th in 2023/24 Thai League 1
- 4-time league winners
- 1-time AFC Cup semi-finalists (2010)
- Returning to AFC club competition for first time since 2011 AFC Cup
- 3rd ACL Two appearance

Dynamic Herb Cebu FC (PHI)
- 2024 Philippines Football League runners-up
- Made AFC club competition debut in 2023/24 AFC Cup
- 2nd ACL Two appearance