Al Wakrah SC (QAT)
- 2024 Qatar Cup winners, 2nd title
- 4th in 2024/24 Qatar Stars League
- 2-time league winners
- Returning to AFC club competition for first time since Asian Club Championship in 2001 and 2002

Tractor FC (IRN)
- 4th in 2023/24 IR Iran league
- 2-time Round of 16 finish in ACL(2016, 2021)
- 1st time in ACL Two

Mohun Bagan Super Giant (IND)
- 2023/24 Indian Super League champions, 1st title
- Reached AFC Cup Inter-zone playoffs twice (2021, 2022)
- 7th ACL Two appearance

FC Ravshan (TJK)
- 2nd in 2023 Tajikistan Higher League
- 2-time league champions
- 5th ACL Two appearance

Al Taawoun FC (KSA)
- 4th in 2023/24 Saudi Pro League
- Reached Round of 16 in 2020 ACL
- 1st time in ACL Two

Air Force SC (IRQ)
- Joint-record 3-time AFC Cup winners
- Unmatched feat of winning all 3 titles back to back
- 2nd in 2023/24 Iraq Stars League
- 7-time league winners
- 4th ACL Two appearance

Al Khaldiya SC (BHR)
- 2023/24 Bahraini Premier League champions, 2nd title and 2nd in a row
- 2nd ACL Two appearance

Altyn Asyr FC (TKM)
- 2023 Turkmenistan Higher League runners-up
- Record 8-time league champions; titles were won back-to-back between 2014 to 2021
- Reached 2018 AFC Cup final, first Turkmen side to do so
- 7th ACL Two appearance

Sepahan SC (IRN)
- AFC Champions League runners-up in 2007
- 2023/24 Hazfi Cup champions
- 3rd in IR Iran league in 2023/24
- 5-time league champions
- 1st time in ACL Two

Sharjah FC (UAE)
- 4th in 2023/24 UAE Pro League
- 6-time league winners
- 1-time ACL quarter-finalists (2004)
- 1st time in ACL Two

FC Istiklol (TJK)
- 2023 Tajikistan Higher League champions, record 12th title
- 2023 Tajikistan Cup winners, record 10th title
- 2-time AFC Cup runners-up in 2015 and 2017
- 7th ACL Two appearance

Al Wehdat (JOR)
- 2023/24 Jordan FA Cup winners, 12th title
- 17-time league champions
- 3-time AFC Cup semi-finalists (2006, 2007, 2011)
- 14th ACL Two appearance

Shabab Al Ahli (UAE)
- 2015 ACL runners-up
- 2023/24 UAE Pro League runners-up
- 8-time league winners
- 1st time in ACL Two

PFC Nasaf (UZB)
- 2023 Uzbekistan Cup winners, 4th title
- 2nd in 2023 Uzbekistan Super League
- 2021 AFC Cup champions
- 4th ACL Two appearance

Al Hussein (JOR)
- 2023/24 Jordanian Pro League champions – first title
- Returning to AFC club competition for first time since 2005 AFC Cup, where they reached quarter-finals
- 2nd ACL Two appearance

Kuwait SC (KUW)
- 2023/24 Kuwaiti Premier League champions – record 19th title
- 3rd in a row and 9th in past 12 seasons
- Joint-record 3-time AFC Cup winners
- 14th ACL Two appearance